Diagnostic Analysis of mZN Microscopy and PCR for Detection of Cryptosporidiosis in Goat Kids - A Molecular Epidemiological Study

The occurrence of Cryptosporidium species infection and its risk factors in neonatal goats is less explored. Also it is due to the fact that diseases like colibacillosis and neonatal viral enteritis complex caused by Group A rotaviruses and Bovine corona viruses can co-exist with Cryptosporidium and can lead to mixed infections and the latter is often overlooked. Therefore, in the current research we explored the cryptosporidial occurrence in neonatal goats of Mathura district of Uttar Pradesh, India. In this study, a total of 644 faecal samples were collected from neonatal goats at different villages and certain organized farms of Mathura district age-wise, season-wise and breed-wise, and were examined for Cryptosporidium based on modified Ziehl-Neelsen technique, conventional 18SSU rRNA nested PCR assay. The overall prevalence of Cryptosporidium infection in goats based on microscopy was 36.80% (237/644;p value <0.0001) and 18SSU rRNA nested PCR 52.95% (341/644;p value <0.0001) respectively. Cryptosporidium species typing was also done using 18SSU rRNA nested PCR-RFLP product using enzymes Mbo-II, Ssp-I and Vsp-I, which revealed species including C. parvum C. bovis, C. ryanae, C. hominis and C. andersoni. Also the infection was clinically associated based on age, gender and seasons to identify the causal relationships that precipitate the cryptosporidial infection in goat kids. Since mZN microscopy based screening requires expertise and may sometimes be confuse with other weak acid fast bodies and also due to low sensitivity, combination of diagnostic tests are used in this study to identify the best test combination that yields best statistical fit in terms of kappa-agreement and McNemar's test. Cryptosporidiosis is caused by an enteric protozoan parasite and the first report in sheep and goat was observed in early 1980s, with other important etiological agents for neonatal diarrhoea, mortality and morbidity in neonatal kids and lambs, responsible for economic losses.Copyright © 2023 Indian Veterinary Assocaition. All rights reserved.

The healthcare experiences of patients with COPD: An IPA study

Introduction/Aim: Studies of the lived experience of Chronic Obstructive Pulmonary Disease (COPD) reveal a number of challenges patients face when interacting with healthcare providers that may be exacerbated by unwillingness or inability to quit smoking. However, none have explored, in-depth, primary care experiences among patients with COPD in community healthcare settings. The study investigated primary care experiences of patients living independently in the community with doctor diagnosed COPD, who smoke or had recently quit smoking and focused on how vulnerability, stigma and emotional components of care shape care experiences. Method(s): An Interpretative Phenomenological Analysis (IPA) involving thirteen participants purposively recruited from social media posts in COPD and carer support groups, general community groups, community noticeboards and paid adverts on social media. In-depth interviews were held between February and April 2022 by phone or Zoom TM and explored healthcare experiences, smoking, stigma and impact of care during COVID-19. Cross-case analysis was conducted to form group experiential themes. Result(s): Participants were aged between 45 to 75 years. Nine were female and two-thirds were current daily smokers. Both positive and negative experiences were explored. Problematic experiences including time-constrained consultations ('You're a number, and that's where I feel like a sheep and [that] can stress you out more'), having to self-advocate for care, and guilt about smoking were common. Positive care experiences described non-judgemental interpersonal interactions with doctors, timely referral, proactive care and trust ('I have an actual great trust for my GP. they're awesome, they'll look after you'). Conclusion(s): Pro-active, empathetic care from GPs is desired by patients living with COPD who continue to smoke but this is not always reflected in the lived experience of care. Stigma and fear of judgement were important underlying drivers of negative care experiences and need to be carefully considered in provision of care for this group.

Summer Meeting of the Anatomical Society

The proceedings contain 71 papers. The topics discussed include: experience of learning human anatomy and histology during COVID-19 pandemic in Kharkiv National Medical University;using musculoskeletal modelling to investigate the functional significance of craniofacial form variation within the genus homo;a morphometric analysis of the cranial fossae in patients with scaphocephaly;exploring the thalamus in young adolescents with psychotic experiences;to replace or not replace that is the question: addressing fate decisions during minipig tooth replacement;anatomy of termination of popliteal artery: a multidetector CT angiographic study;anatomical variation between populations of British red squirrels: the potential impact of supplementary feeding;revealing the biomechanics of the masticatory muscles in the eastern grey squirrel (Sciurus carolinensis) using multibody dynamics analysis;and myoepithelial and immune cell dynamics in the ovine mammary gland during postnatal development.

Susceptibility of different animal species to experimental infection with SARS-CoV-2 (review)

The susceptibility of animal species to SARS-CoV-2, under experimental conditions, is a subject of great interest for the international scientific community. Compared to observational studies of natural disease outbreaks in different animal species, experimental studies based on in silico, in vitro and in vivo research, are important alternatives to evaluate the prediction of potential hosts for SARS-CoV-2 infection. In order to determine the susceptibility of a host species and the risk of acting as a potential animal reservoir, a large number of different animal species, domestic and wild, were experimentally infected with SARS-CoV-2, which were classified as permissive or resistant. Experimental infections have proven to be crucial for clarifying aspects of the pathogenetic mechanism, viral persistence and elimination, immune response, antiviral sensitivity, vaccine production, immunotherapy and improving diagnostic methods. In this article, some experimental infections carried out in different animal species will be reviewed, according to the data from the literature.

Conformity and reactions to deviance in the time of COVID-19

We have seen massive global behavioral change as billions of people radically altered their ways of life in response to COVID-19. Here, we review how research on conformity and deviance can inform understanding of and effective responses to the pandemic. Group identities are critical for understanding who is influenced by whom, as well as how partisan divisions can obstruct cohesive and coordinated action. We identify several questions highlighted by the pandemic, including when people will react more harshly to ingroup members who violate health-protective norms (black sheep effect) or to outgroup members violating the same norms (intergroup hypocrisy). As a working hypothesis, we propose a goal-based approach, positing that differentially negative reactions to ingroup and outgroup deviants are likely influenced by the relative salience of goals to protect ingroup image, enforce group norms, maintain intergroup boundaries, and seek justice for potential victims put at risk by norm-violating behavior. (PsycInfo Database Record (c) 2023 APA, all rights reserved)

Prospect of Vaccinia virus vector vaccine for wildlife

Against a pandemic of emerged infectious disease, COVID-19, new generation vaccines based on nucleic acids or recombinant viruses, which had not been used as vaccines in humans, have been inoculated and shown to be successful. They are, however, heat-labile and need a cold-chain including deep-freezers for storage and transportation. Vaccinia virus (VAC) vector vaccine (VACV) is a pioneer of new generation of vaccines constructed by using molecular biological technology. VACV, which has contributed to eradication of smallpox, has excellent characteristics of vaccinia virus such as a high heat-stability and long-lasting immunological effects. It is possible to distinguish the immunological responses of vaccination from those of natural infections. We started our developmental researches 35 years ago, using attenuated VAC strains established in Japan. In this article, we first describe the early researches of VACVs;development of two VACVs for Bovine leukemia virus and Rinderpest morbillivirus antigens and their protective immunity in large mammals, sheep and cows. Second, application of VACV is described;Rabies-VACV, which has already been licensed, used in the field in Europe and USA, and resulted in a prominent decrease of rabies. Then, current status of VACV research is described;non-replicating VACVs in mammalian cells have been developed as new-generation and ultimately-safe vaccines. We discuss the possibility of future application of VACV for wildlife.

Serum total bilirubin and aminotransferases in patients with COVID-19 in Nellore district of South Andhra Pradesh

BACKGROUND: Corona virus disease-19 (COVID -19) infection is an acute infectious disease caused by a newly discovered beta corona virus, severe acute respiratory syndrome corona virus 2 (SARS-CoV-2). While the primary target organ is the lungs, involvement of many other organs is often evident in patients with COVID-19. There is emerging evidence to suggest association of SARS-CoV-2 infection with development of many liver abnormalities. The purpose of this study was to evaluate the prevalence of abnormal liver parameters in COVID-19 patients and their variation in moderate and severe cases. METHODS: This is a retrospective study. All patients with COVID -19, between the ages 20-75 years, encountered between April and May 2021, were included for the study and compared with age-matched controls. Severity of infection was defined based on the presence of symptoms, oxygen saturation, need for respiratory and intensive care support. Liver parameters such as serum total bilirubin (TBIL), serum aminotransferases, alanine aminotransaminase (ALT) and aspartate aminotransaminase (AST) were analysed. Inflammatory markers such as C-reactive protein (CRP) and D-dimer were also included for assay. RESULTS: A total of 52 patients were encountered during the study period. Of these, 29% (15/52) required intensive care. Abnormal liver parameters were observed in 14 (27%) patients, and were significantly elevated compared to healthy controls. Liver dysfunction was markedly profound in severe infection than those with moderate disease. Higher levels of CRP and D-dimer were noted in severe patients of COVID-19. CONCLUSIONS: Mild liver abnormalities in the form of elevated ALT and AST are seen in COVID-19 patients suggesting mild or no liver injury. These abnormal parameters do not generally lead to significant liver function impairment/failure and no specific treatment is required.

COVID-19 influence on developments in the global beef and sheep sectors

While the COVID-19 pandemic and associated government responses have had a substantial impact on consumers and meat supply chains worldwide, the effect on beef and sheep farming has been surprisingly small, short-lived and largely offset by other global influences. However, the impact has also varied greatly between countries and regions, largely due to differences in Government measures and in industry circumstances and influences. This study aims to provide insights into the pandemic's impacts throughout global beef and sheep supply chains, but with a focus on the farm level, particularly producer prices in 2020. At the centre of the study is an analysis of online questionnaire-based survey responses to the Global agri benchmark Beef and Sheep Network. The study also utilizes a variety of other studies and information sources to explore other potential factors that could have also driven beef and sheep sectors worldwide in 2020. It explores how these influences interacted with the effect of the pandemic. Food service sales were highly impacted by the pandemic, meat processing was temporarily disrupted in North America but global livestock prices remained high due, in large part, to the continuation of strong beef and sheep meat demand and imports in China.

Stability of SARS-CoV-2 in Biological Fluids of Animals.

Since its first emergence in 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has continued to evolve genetically, jump species barriers, and expand its host range. There is growing evidence of interspecies transmission including infection of domestic animals and widespread circulation in wildlife. However, knowledge of SARS-CoV-2 stability in animal biological fluids and their role in transmission is still limited as previous studies focused on human biological fluids. Therefore, this study aimed to determine the SARS-CoV-2 stability in biological fluids from three animal species, cats, sheep and white-tailed deer (WTD). Saliva, feces, 10% fecal suspensions, and urine of cats, sheep, and WTD were mixed with a known concentration of virus and incubated under indoor and three different climatic conditions. Our results show that the virus was stable for up to 1 day in the saliva of cats, sheep, and WTD regardless of the environmental conditions. The virus remained infectious for up to 6 days in feces and 15 days in fecal suspension of WTD, whereas the virus was rather unstable in cat and sheep feces and fecal suspensions. We found the longest survival of SARS-CoV-2 in the urine of cats, sheep, and WTD. Furthermore, side-by-side comparison with different SARS-CoV-2 strains showed that the Alpha, Delta, and Omicron variants of concern were less stable than the ancestral Wuhan-like strain in WTD fecal suspension. The results of our study provide valuable information for assessing the potential role of various animal biological fluids in SARS-CoV-2 transmission.

First serological evidence of SARS-CoV-2 natural infection in small ruminants : Brief report.

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) emerged in late December 2019 and spread worldwide, quickly becoming a pandemic. This zoonotic coronavirus shows a broad host range, including wildlife and domestic animals. Small ruminants are shown to be susceptible to SARS-CoV-2 but, to date, no natural infection has been reported. Herein, we performed a survey for SARS-CoV-2 among sheep and goats in the Campania region of Italy using an indirect multispecies ELISA. Next, positive sera were submitted to virus serum neutralization for the quantification of specific neutralizing antibodies. Out of 612 sheep and goats, 23 were found ELISA positive (3.75%) and 1 of them showed 1:20 neutralizing antibodies titer. No significant difference was found between the two species, as well as between male and female, geographical location and age. Our findings demonstrate that natural infection can occur in flocks in a field situation. Moreover, low susceptibility to SARS-CoV-2 is reported for sheep and goats, nevertheless, the continuous mutations of this virus open new scenarios on viral host range and tropism, highlighting the importance of investigating animal species that could represent ongoing or future possible hosts.

COVID Masks as Semiotic Expressions of Hate.

In April 2021, as COVID briefly appeared to recede in the United States, Fox News host Tucker Carlson went on a lengthy rant against mask wearers. It appeared as if, to paraphrase Hegel, the owl of Minerva was flying at dusk. Why complain about masks at the very time mask mandates were being rolled back and society was-or so it seemed-returning to normal? The answer must lie in the mask itself, and what it represents. In anti-masking discourse, the mask has had two symbolic meanings-mask wearers as sheep, and the masks as burqas. Sheep are obedient, while burqas are instruments of social control. At a deeper level, the very act of mask wearing becomes seen as oppressive, while revealing one's face is freedom itself. This view of masking (and revealing one's face) is not new, rather it dates back in Europe to a "revolutionary transparency" that emerged in the wake of the French Revolution that has been appropriated by anti-maskers. While the sheep and burqa images have some play in anti-masking discourse, the connection between freedom and showing one's face is the most durable message anti-maskers see conveyed by the COVID face mask.

The experience of primary care for patients with chronic obstructive pulmonary disease (COPD) who continue to smoke: an interpretative phenomenological inquiry

Background: Studies of adults living with Chronic Obstructive Pulmonary Disease (COPD) reveal a number of challenges when interacting with healthcare providers and may be exacerbated by unwillingness or inability to quit smoking. However, none have explored in-depth, primary care experiences among patients with COPD in the Australian healthcare setting. Aim/Objectives: The study used Interpretive Phenomenological Analysis (IPA) to explore healthcare experiences of participants in primary care settings amongst current smokers or those who had recently quit smoking. Method(s): The study was guided by principles of IPA and participants' sense-making of their personal and social 'world', particular experiences, events and emotions were explored. Thirteen participants were purposively recruited from 33 responses to social media posts in COPD and carer support groups, general community groups, community noticeboards and paid adverts. In-depth interviews were held between February and April 2022 by phone or Zoom. Interviews explored healthcare experiences, smoking and stigma. Cross-case analysis was conducted to form group experiential themes. Finding(s): Participants self-reported doctor diagnosed COPD, were living in Australia and aged between 45 to 75 years. Nine were female and 60% were current smokers. Interviews explored experiences of care including access, interpersonal care, coordination, comprehensiveness of services and trust. Participants described how their care experience shifted as primary care adapted care delivery during COVID. Problematic experiences included timeconstrained consultations 'You're a number, and that's where I feel like a sheep and they can stress you out more', having to self-advocate for care and guilt about smoking. Positive care experiences described non-judgemental interpersonal interactions with doctors, timely referral, proactive care and trust 'I have an actual great trust for my GP they're awesome, they'll look after you'. Implications: Understanding and valuing the patient experience in healthcare interactions provides insights to how care can be transformed for vulnerable patients living with socially stigmatised illness.

ASAIO 2022 Annual Meeting Abstracts

The proceedings contain 226 papers. The topics discussed include: identification of biomarkers sensitive to pulsatile and continuous flow for identification of promising continuous flow VAD modulation protocols to mitigate non-surgical bleeding events;comprehensive machine learning analysis of pre-implantation risk factors for right heart failure after LVAD implantation;combining VA-ECMO And Impella (EC-Pella) before reperfusion mitigates left ventricular loading and injury due to VA-ECMO in acute myocardial infarction;platelet function at the intersection of the COVID-19 'cytokine storm' and mechanical circulatory support;a dialysate free portable artificial kidney device;durable right heart mechanical support system: a multi-day proof-of-concept study in pulmonary hypertension sheep;a dual-action nitric oxide-releasing slippery surface coating for extracorporeal organ support: first evaluation at clinically relevant blood flow rate for partial lung support;cannula add-on for pressure and flow measurement in VADs;and comparison of interlaboratory CFD simulations of the FDA benchmark blood pump model.

The role of sheep's milk bioactive substances in the prevention of metabolic and viral diseases

Sheep's milk exerts a number of biological effects that influence metabolic reactions and disease resistance. Sheep's milk contains the highest level of casein, whey protein and conjugated linoleic acid isomers (CLA), which stimulate the immune system, and have "anti-obesity", "antidiabetic" and "anticancer" properties. Sheep's milk is an excellent source of protein and group B vitamins, which ensure the proper functioning of the nervous system. Proline-rich polypeptide partially reverses neurodegenerative changes and has immunoregulatory and pro-cognitive properties. CLA isomers also reduce oxidative stress and mitochondrial dysfunction in the brain, which may be important in neurodegenerative diseases such as Alzheimer's disease. Sheep's milk shows the highest inhibitory activity of angiotensin converting enzyme, which is crucial in preventing SARS-CoV-2 infection. Systemic inflammation is a common factor in atherosclerosis and COVID-19. Polar lipids present in sheep's milk lower inflammatory biomarkers and reduce the development of atherosclerosis. Therefore, dairy products can be used to help prevent COVID-19 disease. Sheep's milk and its products containing bioactive substances may be promising ingredients for the production of health-promoting functional foods.

Molecular survey for tick-borne pathogens and associated risk factors in sheep and goats in Kano Metropolis, Nigeria.

Tick-borne pathogens (TBPs) pose an increased health and productivity risk to livestock in sub-Saharan Africa. Information regarding TBPs infecting small ruminants in Kano metropolis is scarce. Therefore, we investigated the molecular epidemiology of tick-borne pathogens of economic importance from sheep and goats in Kano, Nigeria using Polymerase chain reaction (PCR). A total of 346 blood DNA samples were collected from small ruminants and analyzed for TBPs using PCR and sequencing. Risk of infection was determined for age, sex, breed and animal species. Our results indicate the absence of piroplasmids (Babesia/Theileria) and Rickettsia spp. infections. The overall prevalence for Anaplasma spp. was 9.25% (32/346) with a higher prevalence in goats 13.59% (25/184) compared with sheep 4.32% (7/162). With respect to age of animals, goats >4 years had the highest prevalence of 32.45% (11/37) which differs significantly (P = 0.0059) compared with other age categories. Cross breed goats had a prevalence of 15.63% (5/32) compared with Kano brown breed 14.08 (20/142). Sex significant difference (P = 0.029) was observed in the goats with females having the highest prevalence 20.89% (14/67) compared with males 9.40% (11/117). Furthermore, with regards to sheep, no significant difference (P > 0.05) was observed with respect to age and breed. Finally, no significant difference (P > 0.05) was observed with the prevalence of Anaplasma spp. due to Body condition score (BCS) in both sheep and goats. Conclusively, the occurrence of TBPs in small ruminants is low. Continuous efforts in tick control must be sustained to ensure high productive yield and reduced disease burden associated with TBPs of sheep and goats in Kano metropolis.

First person – Mugagga Kalyesubula and Ramgopal Mopuri

First Person is a series of interviews with the first authors of a selection of papers published in Disease Models & Mechanisms, helping early-career researchers promote themselves alongside their papers. Mugagga Kalyesubula and Ramgopal Mopuri are co-first authors on ‘High-dose vitamin B1 therapy prevents the development of experimental fatty liver driven by overnutrition’, published in DMM. Mugagga is a PhD student in the lab of Dr Hay Dvir at the Volcani Center – Agricultural Research Organization (ARO), Rishon LeZion, Israel and The Hebrew University of Jerusalem, Rehovot, Israel, investigating therapy development for chronic metabolic diseases such as non-alcoholic fatty liver disease. Ramgopal is a postdoctoral researcher in the lab of Dr Hay Dvir at the Volcani Center – ARO in Rishon LeZion, Israel, developing therapeutic approaches for the management of fatty liver diseases.

Suspect narratives: “Sinifying” an “Indianized” Japanese story

In 2013, the Malayalam film Drishyam, a suspenseful story of the cover up of an accidental murder, became a huge hit in India that inspired remakes in many regional languages including one in Hindi that, as with other recent Bollywood hits, traveled to China. This time, though, instead of screening the Hindi film in theaters, the narrative reached Chinese audiences with a Chinese language remake, titled Sheep Without A Shepherd《误杀》. The original film has been accused of lifting its story from a popular Japanese detective novel, The Devotion of Suspect X, which was also made into films in Japanese, Chinese, and Korean. This essay traces the many versions of the narrative to explore how comparing the Indian and Chinese films can recenter our understanding of global cinema and film circulation. When considering the many version of Drishyam, instead of focusing on tensions between center and periphery, we can examine both the anxieties and the creative power of cultural borrowing and the retelling of narratives in an increasingly inter-connected Asian film market

Quarterly report of investigations of suspected exotic diseases: July to September 2021

Exotic pest and disease investigations are managed and reported by the Ministry for Primary Industries' (MPI's) Diagnostic and Surveillance Directorate. This article presents a summary of investigations of suspect exotic and emerging pests and diseases in New Zealand during the period from July to September 2021.

Coronavirus infections in large and small ruminants

Coronavirus infections rank first in the pathology of the gastrointestinal tract and respiratory system in large and small ruminants. The paper analyzes the occurrence, spread, development, trends and prevention of coronavirus infections in ruminants in Bulgaria, Europe and around the world. Retrospective, descriptive and statistical methods were used. The experience and the specifics of the coronavirus diseases encountered so far in large and small ruminants - cattle, buffaloes, camels, deer, sheep and goats and their epizootic character are summarized.